Writing Assignment help on : A Research Work on Apoptosis and Survivin
This article is devised to have an insight about the role of survivin, a protein of inhibitor of apoptosis family, in fighting the cancer. Apoptosis is a natural activity of our body in which our cells follow a typical pattern to kill themselves, thus saving us from those fatal cells which could lead to diseases. Survivin is known for its two functions- suppression of apoptosis and regulation of cell division. Recent studies have shown that over-expressed survivin has exhibited a protective action against the drug-induced apoptosis, which in many cases, lead to breast and other types of cancer. The results have further demonstrated that if the movement of survivin is restricted only to nucleus of cells, the impact of chemotherapeutic drug on a cancer patient is most effective (S. Fukuda and L. M. Pelus 2006).
Four methods are used for this study – Tissue Culture is used to generate the cancer cells in laboratory as the cancer cells have the tendency to divide the other normal cells resulting in the formation of tumour (ehow 2012). Substances used were BT20, MDA-MB-231, MDA-MB-468, 293Twith Invitrogen solution. Sub-cellular Fractionation enables protein localization assessment and protein enrichment from specific cellular compartments. Contents used for this are hypotonic buffer; Tris–Cl pH 7.5, NaCl, MgCl2, 1:100 PIC (Protease Inhibitor Cocktail)
Immuno-blotting is used to check-out some important proteins in a sample of a tissue containing homogenate and antibodies employed here are : 1/1000 rabbit anti-Apaf-1, 1/1000 rabbit anti-Histone H3 and 1/1000 mouse anti-Survivin. Over-expression of survivin employs substances such as QIAGEN QIA prep-purified pc DNA 3.1 vectors which encoding include GFP, survivin-GFP etc. using Lipofectamine (Bioscience Horizons 2008; ehow 2012).
The search for new therapeutic drug to control the ill-effects of unregulated apoptosis gained importance as many doctors feared that unregulated apoptosis could lead to several diseases including cancer, neurodegenerative diseases, autoimmune disorders, and AIDS. A very important beneficiary of apoptosis research is oncology, since most cancer cells exhibit defects in their suicidal machinery (EC Lacasse, S Baird, RG Korneluketal.1998). If researchers take serious interest in deregulated apoptosis, new ways could be developed to kill the cancerous cells in the body (QL Deveraux, N Roy, HR Stennicke et al. 1998).
The main discovery of this research work by Helen Angell highlights that over-expression of survivin can be useful in treatment of breast-cancer as it shields breast-cancer cells from apoptosis usually a side-effect of some chemotherapeutic drugs. The only requirement is that survivin must be present within cytosol.
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